• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用二硫键交联来探究一种设计的四螺旋束蛋白的结构和柔韧性。

Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein.

作者信息

Regan L, Rockwell A, Wasserman Z, DeGrado W

机构信息

Experimental Station, E.I. du Pont de Nemours and Company, Wilmington, Delaware 19880, USA.

出版信息

Protein Sci. 1994 Dec;3(12):2419-27. doi: 10.1002/pro.5560031225.

DOI:10.1002/pro.5560031225
PMID:7756995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2142780/
Abstract

The introduction of disulfide crosslinks is a generally useful method by which to identify regions of a protein that are close together in space. Here we describe the use of disulfide crosslinks to investigate the structure and flexibility of a family of designed 4-helix bundle proteins. The results of these analyses lend support to our working model of the proteins' structure and suggest that the proteins have limited main-chain flexibility.

摘要

引入二硫键交联是一种普遍有用的方法，可用于确定蛋白质中在空间上彼此靠近的区域。在此，我们描述了利用二硫键交联来研究一类设计的四螺旋束蛋白的结构和柔韧性。这些分析结果支持了我们关于该蛋白质结构的工作模型，并表明这些蛋白质的主链柔韧性有限。

相似文献

1
Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein.利用二硫键交联来探究一种设计的四螺旋束蛋白的结构和柔韧性。
Protein Sci. 1994 Dec;3(12):2419-27. doi: 10.1002/pro.5560031225.
2
Global topology & stability and local structure & dynamics in a synthetic spin-labeled four-helix bundle protein.合成自旋标记四螺旋束蛋白中的全局拓扑结构与稳定性以及局部结构与动力学
Biochemistry. 1997 Mar 11;36(10):2798-806. doi: 10.1021/bi9618225.
3
Effect of four helix bundle topology on heme binding and redox properties.四螺旋束拓扑结构对血红素结合及氧化还原性质的影响。
Biochemistry. 1998 Mar 31;37(13):4635-43. doi: 10.1021/bi971856s.
4
Conformational studies of a bombolitin III-derived peptide mimicking the four-helix bundle structural motif of proteins.一种模仿蛋白质四螺旋束结构基序的蜂毒素III衍生肽的构象研究。
J Am Chem Soc. 2003 Dec 17;125(50):15314-23. doi: 10.1021/ja0300970.
5
Contribution of a single-turn alpha-helix to the conformational stability and activity of the alkaline proteinase inhibitor of Pseudomonas aeruginosa.单圈α-螺旋对铜绿假单胞菌碱性蛋白酶抑制剂构象稳定性和活性的贡献。
Biochemistry. 2005 Feb 22;44(7):2469-77. doi: 10.1021/bi048287q.
6
Control of lysine reactivity in four-helix bundle proteins by site-selective pKa depression: expanding the versatility of proteins by postsynthetic functionalization.通过位点选择性pKa降低控制四螺旋束蛋白中赖氨酸的反应性：通过合成后功能化扩展蛋白质的多功能性。
Chemistry. 2002 Aug 16;8(16):3687-97. doi: 10.1002/1521-3765(20020816)8:16<3687::AID-CHEM3687>3.0.CO;2-8.
7
Analysis of peptide design in four-, five-, and six-helix bundle template assembled synthetic protein molecules.四螺旋、五螺旋和六螺旋束模板组装合成蛋白分子中的肽设计分析。
Biopolymers. 2007;88(5):774-9. doi: 10.1002/bip.20791.
8
Synthesis and conformational properties of protein fragments based on the Id family of DNA-binding and cell-differentiation inhibitors.基于DNA结合及细胞分化抑制因子Id家族的蛋白质片段的合成与构象特性
Biopolymers. 2005;80(6):762-74. doi: 10.1002/bip.20287.
9
Solution structure of a de novo helical protein by 2D-NMR spectroscopy.利用二维核磁共振光谱法测定的一种全新螺旋蛋白的溶液结构。
J Mol Biol. 1994 Feb 25;236(3):862-8. doi: 10.1006/jmbi.1994.1194.
10
Min-21 and min-23, the smallest peptides that fold like a cystine-stabilized beta-sheet motif: design, solution structure, and thermal stability.Min-21和min-23，折叠方式类似胱氨酸稳定β-折叠基序的最小肽：设计、溶液结构及热稳定性
Biochemistry. 1999 Aug 10;38(32):10615-25. doi: 10.1021/bi990821k.

引用本文的文献

1
protein design, a retrospective.蛋白质设计：回顾
Q Rev Biophys. 2020 Feb 11;53:e3. doi: 10.1017/S0033583519000131.
2
Controlled protein dimerization through hybrid coordination motifs.通过混合配位基序控制蛋白质二聚化。
Inorg Chem. 2010 May 3;49(9):4362-9. doi: 10.1021/ic100534y.
3
Use of thiol-disulfide equilibria to measure the energetics of assembly of transmembrane helices in phospholipid bilayers.利用硫醇-二硫键平衡来测量磷脂双层中跨膜螺旋组装的能量学。
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14772-7. doi: 10.1073/pnas.2536751100. Epub 2003 Dec 1.
4
Determination of membrane protein stability via thermodynamic coupling of folding to thiol-disulfide interchange.通过折叠与硫醇-二硫键交换的热力学偶联来测定膜蛋白稳定性
Protein Sci. 2003 Aug;12(8):1732-40. doi: 10.1110/ps.0378603.
5
A single disulfide bond restores thermodynamic and proteolytic stability to an extensively mutated protein.单个二硫键可恢复一个发生广泛突变的蛋白质的热力学稳定性和蛋白酶解稳定性。
Protein Sci. 2000 Sep;9(9):1642-50. doi: 10.1110/ps.9.9.1642.
6
Introduction of a [4Fe-4S (S-cys)4]+1,+2 iron-sulfur center into a four-alpha helix protein using design parameters from the domain of the Fx cluster in the Photosystem I reaction center.利用光系统I反应中心中Fx簇结构域的设计参数，将一个[4Fe-4S (S-半胱氨酸)4]+1,+2铁硫中心引入到一个四α螺旋蛋白中。
Protein Sci. 1997 Feb;6(2):340-6. doi: 10.1002/pro.5560060209.
7
Surface point mutations that significantly alter the structure and stability of a protein's denatured state.显著改变蛋白质变性状态的结构和稳定性的表面点突变。
Protein Sci. 1996 Oct;5(10):2009-19. doi: 10.1002/pro.5560051007.

本文引用的文献

1
Metal ion-dependent modulation of the dynamics of a designed protein.金属离子依赖性对一种设计蛋白质动力学的调节
Science. 1993 Aug 13;261(5123):879-85. doi: 10.1126/science.8346440.
2
Disulfide bonds and the stability of globular proteins.二硫键与球状蛋白质的稳定性
Protein Sci. 1993 Oct;2(10):1551-8. doi: 10.1002/pro.5560021002.
3
One-disulfide intermediates of apamin exhibit native-like structure.蜂毒明肽的单二硫键中间体呈现出类似天然的结构。
Biochemistry. 1994 May 3;33(17):5253-61. doi: 10.1021/bi00183a031.
4
Kinetic role of a meta-stable native-like two-disulphide species in the folding transition of bovine pancreatic trypsin inhibitor.亚稳态天然样双二硫键物种在牛胰蛋白酶抑制剂折叠转变中的动力学作用
J Mol Biol. 1984 Nov 5;179(3):497-526. doi: 10.1016/0022-2836(84)90077-9.
5
Acetamidomethyl. A novel thiol protecting group for cysteine.乙酰氨基甲基。一种用于半胱氨酸的新型硫醇保护基团。
J Am Chem Soc. 1972 Jul 26;94(15):5456-61. doi: 10.1021/ja00770a600.
6
Computer-aided model-building strategies for protein design.
Biochemistry. 1986 Oct 7;25(20):5987-91. doi: 10.1021/bi00368a023.
7
An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of lambda repressor.
Biochemistry. 1986 Oct 7;25(20):5992-8. doi: 10.1021/bi00368a024.
8
In vivo formation and stability of engineered disulfide bonds in subtilisin.枯草杆菌蛋白酶中工程二硫键的体内形成与稳定性
J Biol Chem. 1986 May 15;261(14):6564-70.
9
Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation.具有盐酸胍和尿素变性模式改变的葡萄球菌核酸酶突变体形式。
Proteins. 1986 Sep;1(1):81-9. doi: 10.1002/prot.340010113.
10
An engineered disulfide bond in dihydrofolate reductase.
Biochemistry. 1987 Apr 21;26(8):2182-9. doi: 10.1021/bi00382a017.