Miura Y, Hamada A, Utsumi H
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Free Radic Res. 1995 Mar;22(3):209-14. doi: 10.3109/10715769509147540.
In vivo antioxidant activity seems to be quite complicate due to multiple interaction with biomaterials and differs from results by in vitro experiments. In vivo estimation of antioxidant activity is performed by measuring TBA reactive substances in blood or hydrocarbon gases in breath, but these systems do not measure free radical reaction but the final products of oxidative reaction. In the present study, we applied in vivo ESR to evaluate antioxidant activity by monitoring the redox reaction of nitroxide radical and clearly found that the nitroxide is very susceptible to oxidative stress in vivo and quite useful to evaluate antioxidant activity non-invasively.
由于与生物材料的多种相互作用，体内抗氧化活性似乎相当复杂，并且与体外实验的结果不同。通过测量血液中的TBA反应性物质或呼出气体中的碳氢化合物气体来进行体内抗氧化活性的评估，但这些系统测量的不是自由基反应，而是氧化反应的最终产物。在本研究中，我们应用体内电子自旋共振通过监测氮氧自由基的氧化还原反应来评估抗氧化活性，并且清楚地发现氮氧自由基在体内对氧化应激非常敏感，并且对于非侵入性评估抗氧化活性非常有用。
