Ogose A, Motoyama T, Hotta T, Watanabe H, Takahashi H E
Department of Pathology, Niigata University School of Medicine, Japan.
Virchows Arch. 1995;426(2):117-25. doi: 10.1007/BF00192632.
Osteosarcomas contain variable amounts of bony tissue, but the mechanism of bone formation by osteosarcoma is not well understood. While a number of cultured human osteosarcoma cell lines have been established, they are maintained by different media and differ qualitatively with regard to bone formation. We examined different media for their ability to support bone formation in vitro and found the alpha-modification of Eagle's minimal essential medium supplemented with beta glycerophosphate was best for this purpose, because it contained the proper calcium and phosphate concentrations. Subsequently, we compared seven human osteosarcoma cell lines under the same experimental conditions to clarify their ability to induce bone formation. NOS-1 cells most frequently exhibited features of bone formation in vitro and in nude mice. Collagen synthesis by tumour cells themselves seemed to be the most important factor for bone volume. However, even HuO9 cells, which lacked collagen synthesis and failed to form bone in vitro, successfully formed tumours containing bone in nude mice. Histological analysis of HuO9 cells in diffusion chambers implanted in nude mice and the findings of polymerase chain reaction indicated that the phenomenon was probably due to bone morphogenetic protein.
骨肉瘤含有数量不等的骨组织，但骨肉瘤形成骨的机制尚未完全清楚。虽然已经建立了多种培养的人骨肉瘤细胞系，但它们是在不同培养基中培养的，并且在骨形成方面存在质的差异。我们研究了不同培养基支持体外骨形成的能力，发现补充了β-甘油磷酸的伊格尔氏最低必需培养基的α-改良型最适合此目的，因为它含有合适的钙和磷浓度。随后，我们在相同实验条件下比较了七种人骨肉瘤细胞系，以阐明它们诱导骨形成的能力。NOS-1细胞在体外和裸鼠中最常表现出骨形成的特征。肿瘤细胞自身的胶原蛋白合成似乎是骨量的最重要因素。然而，即使是缺乏胶原蛋白合成且在体外不能形成骨的HuO9细胞，在裸鼠中也成功形成了含骨的肿瘤。对植入裸鼠扩散室中的HuO9细胞进行组织学分析以及聚合酶链反应结果表明，这种现象可能是由于骨形态发生蛋白所致。
