Lugo N, Kicliter E
Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan.
Brain Res. 1995 Feb 27;673(1):161-4. doi: 10.1016/0006-8993(94)01462-q.
Bilaterally projecting retinal ganglion cells have recently been reported in the Japanese monkey Macaca fuscata and albino rat. In the present experiments, we sought to determine whether ganglion cells project bilaterally in the 13-lined ground squirrel Spermophilus tridecemlineatus. We injected rhodamine-conjugated latex microspheres (red beads) into the right optic tract, superior colliculus and the dorsal lateral geniculate nucleus and FITC-conjugated latex microspheres (green beads) into these structures on the left side. In retinal wholemounts of all subjects, we found cells filled with red beads throughout the left retina and cells with green beads throughout the right retina. Ipsilaterally projecting ganglion cells were confined to the temporal retina where they were mixed with cells projecting contralaterally. In no case did we observe a doubly labeled cell. We interpret these observations as indicating that ganglion cells in this species do not project bilaterally.
最近有报道称，日本猕猴和白化大鼠中存在双侧投射的视网膜神经节细胞。在本实验中，我们试图确定在十三条纹地松鼠中神经节细胞是否进行双侧投射。我们将罗丹明偶联的乳胶微球（红色珠子）注入右侧视束、上丘和背外侧膝状核，并将异硫氰酸荧光素偶联的乳胶微球（绿色珠子）注入左侧的这些结构。在所有受试动物的视网膜整装标本中，我们在整个左侧视网膜中发现了充满红色珠子的细胞，在整个右侧视网膜中发现了充满绿色珠子的细胞。同侧投射的神经节细胞局限于颞侧视网膜，在那里它们与对侧投射的细胞混合在一起。我们在任何情况下都未观察到双标记细胞。我们将这些观察结果解释为表明该物种的神经节细胞不会进行双侧投射。
