维生素D类似物对MCF-7人乳腺癌细胞的抗增殖作用。

The antiproliferative effect of vitamin D analogs on MCF-7 human breast cancer cells.

作者信息

Brenner R V, Shabahang M, Schumaker L M, Nauta R J, Uskokovic M R, Evans S R, Buras R R

机构信息

Department of Surgery, Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, DC 20007-2197, USA.

出版信息

Cancer Lett. 1995 May 25;92(1):77-82. doi: 10.1016/0304-3835(95)03765-o.

DOI:10.1016/0304-3835(95)03765-o

PMID:7757962

Abstract

We analyzed the antiproliferative effect of 1,25-dihydroxyvitamin D3 and four vitamin D analogs on MCF-7, a human breast cancer cell line known to express the vitamin D receptor. Growth curve studies and [3H]thymidine incorporation assays were used to assess the antiproliferative effect of 1,25-dihydroxyvitamin D3 (vitamin D), Ro 23-7553, Ro 24-5531, Ro 25-5317, and Ro 24-5583. Growth of MCF-7 cells was significantly inhibited by 1,25-dihydroxyvitamin D3 and all four analogs at 10(-8) M (P < 0.05). MCF-7 cells treated with analog had significantly less [3H]thymidine incorporation than cells treated with 1,25-dihydroxyvitamin D3 (P < 0.05). The affinity of the analogs for the vitamin D receptor was similar to that of 1,25-dihydroxyvitamin D3. These results demonstrate that analogs of 1,25-dihydroxyvitamin D3 are potent antiproliferative agents on human breast cancer cells and that this activity is likely mediated through the vitamin D receptor.

摘要

我们分析了1,25 - 二羟基维生素D3和四种维生素D类似物对MCF - 7的抗增殖作用，MCF - 7是一种已知表达维生素D受体的人乳腺癌细胞系。生长曲线研究和[3H]胸苷掺入试验用于评估1,25 - 二羟基维生素D3（维生素D）、Ro 23 - 7553、Ro 24 - 5531、Ro 25 - 5317和Ro 24 - 5583的抗增殖作用。在10(-8) M浓度下，1,25 - 二羟基维生素D3和所有四种类似物均显著抑制MCF - 7细胞的生长（P < 0.05）。用类似物处理的MCF - 7细胞的[3H]胸苷掺入量显著低于用1,25 - 二羟基维生素D3处理的细胞（P < 0.05）。这些类似物对维生素D受体的亲和力与1,25 - 二羟基维生素D3相似。这些结果表明，1,25 - 二羟基维生素D3的类似物是对人乳腺癌细胞有效的抗增殖剂，并且这种活性可能是通过维生素D受体介导的。