Bayer P M, Zazgornik J, Schmidt P, Bratusch-Marrein P, Gabl F
Wien Klin Wochenschr. 1975 Oct 17;87(19):646-8.
The total activity and the isoenzyme fractions of alkaline phosphatase were determined in the serum of 15 patients on intermittent haemodialysis and 32 kidney transplant recipients. The isoenzymes of alkaline phosphatase were separated by polyacrylamide gel disc electrophoresis. It was apparent that the increased activity of serum alkaline phosphatase in these patients was not limited to a distinct isoenzyme fraction. 5 of the 15 dialysis patients and 19 (59%) of the 32 kidney transplant patients showed a bone-type isoenzyme pattern. The possible causes for the presence of a bone-type alkaline phosphatase pattern are discussed. The fractionation of alkaline phosphatase gives a valuable indication as to the organ of origin of the particular fraction and is also of diagnostic value when the total activity of alkaline phosphatase lies within the normal range.
测定了15例间歇性血液透析患者和32例肾移植受者血清中碱性磷酸酶的总活性及同工酶组分。采用聚丙烯酰胺凝胶圆盘电泳法分离碱性磷酸酶同工酶。显然，这些患者血清碱性磷酸酶活性的升高并不局限于某一特定的同工酶组分。15例透析患者中有5例，32例肾移植患者中有19例（59%）呈现骨型同工酶模式。讨论了出现骨型碱性磷酸酶模式的可能原因。碱性磷酸酶的分级分离对于特定组分的起源器官提供了有价值的指示，并且当碱性磷酸酶的总活性在正常范围内时也具有诊断价值。
