Guo S, Kemphues K J
Section of Genetics and Development, Cornell University, Ithaca, New York 14853, USA.
Cell. 1995 May 19;81(4):611-20. doi: 10.1016/0092-8674(95)90082-9.
The first cleavage of C. elegans is asymmetric, generating daughter cells with different sizes, cytoplasmic components, and fates. Mutations in the par-1 gene disrupt this asymmetry. We report here that par-1 encodes a putative Ser/Thr kinase with similarity to kinases from yeasts and mammals. Two strong alleles have mutations in the kinase domain, suggesting that kinase activity is essential for par-1 function. PAR-1 protein is localized to the posterior periphery of the zygote and is distributed in a polar fashion preceding the asymmetric divisions of the germline lineage. Because PAR-1 distribution in the germline correlates with the distribution of germline-specific P granules, it is possible that PAR-1 functions in germline development as well as in establishing embryonic polarity.
秀丽隐杆线虫的第一次卵裂是不对称的，产生大小、细胞质成分和命运不同的子细胞。par-1基因的突变会破坏这种不对称性。我们在此报告，par-1编码一种推定的丝氨酸/苏氨酸激酶，与酵母和哺乳动物的激酶相似。两个强等位基因在激酶结构域发生突变，表明激酶活性对par-1功能至关重要。PAR-1蛋白定位于受精卵的后周边，并在种系谱系的不对称分裂之前以极性方式分布。由于种系中PAR-1的分布与种系特异性P颗粒的分布相关，因此PAR-1可能在种系发育以及建立胚胎极性中发挥作用。
