Kupcsulik P, Flautner L, Bodnár A
Acta Chir Acad Sci Hung. 1975;16(2):183-201.
The ischaemic lesion of the pancreaticoduodenal graft prepared for transplantation was studied by enzyme histochemical methods. Estimation of lactate dehydrogenase, non-specific acid and alkaline phosphatases and esterases and of glucose-6-phosphatase pancreas. While in the pancreas, lesions due to warm ischaemia will appear after one hour, the same can be observed in the duodenum after 30 minutes and in both organs the lesions progress rapidly. Lesions resulting from cold effects appear in the pancreas after three hours and remain unchanged for 15 to 18 hours, while in the duodenum they will appear after one hour and deteriorate considerably after three hours. Continuous perfusion for more than 30 minutes or the glucose perfusion test performed after cold ischaemia of more than two hours duration considerably affected the enzymes of the organs.
采用酶组织化学方法研究了用于移植的胰十二指肠移植物的缺血性病变。测定了乳酸脱氢酶、非特异性酸性和碱性磷酸酶、酯酶以及葡萄糖-6-磷酸酶在胰腺中的含量。虽然在胰腺中，热缺血导致的损伤在1小时后就会出现，但十二指肠在30分钟后也会出现同样的情况，并且两个器官中的损伤进展迅速。冷效应导致的损伤在胰腺中3小时后出现，并在15至18小时内保持不变，而在十二指肠中，它们在1小时后出现，并在3小时后显著恶化。持续灌注超过30分钟或在持续超过两小时的冷缺血后进行葡萄糖灌注试验，会对器官的酶产生显著影响。
