Hintz N J, Ennis D G, Liu W F, Larsen S H
Department of Microbiology and Immunology, School of Medicine, Indiana University, Indianapolis 46202-5120, USA.
FEMS Microbiol Lett. 1995 Apr 1;127(3):175-80. doi: 10.1111/j.1574-6968.1995.tb07470.x.
The recA gene of Chlamydia trachomatis was isolated by complementation of an Escherichia coli recA mutant. The cloned gene restored resistance to methyl methanesulfonate in E. coli recA mutants. The DNA sequence of the chlamydial gene was determined and the deduced protein sequence compared with other RecA proteins. In E. coli recA deletion mutants, the cloned gene conferred moderate recombinational activity as assayed by Hfr matings. The chlamydial recA gene was efficient in repairing alkylated DNA but less so in repairing of UV damage when compared with the E. coli homologue. As detected by an SOS gene fusion, a small but measurable amount of LexA co-cleavage was indicated.
沙眼衣原体的recA基因是通过对大肠杆菌recA突变体进行互补分离得到的。克隆的基因恢复了大肠杆菌recA突变体对甲磺酸甲酯的抗性。测定了衣原体基因的DNA序列，并将推导的蛋白质序列与其他RecA蛋白进行了比较。在大肠杆菌recA缺失突变体中，通过高频重组（Hfr）交配分析，克隆的基因赋予了中等程度的重组活性。与大肠杆菌同源物相比，衣原体recA基因在修复烷基化DNA方面效率较高，但在修复紫外线损伤方面效率较低。通过SOS基因融合检测，发现有少量但可测量的LexA共切割现象。
