玉米中两对类AGAMOUS MADS-box基因的结构表征、染色体定位及系统发育评估

Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize.

作者信息

Theissen G, Strater T, Fischer A, Saedler H

机构信息

Max-Planck-Institut für Züchtungsforschung, Abteilung Molekulare Pflanzengenetik, Köln, Germany.

出版信息

Gene. 1995 Apr 24;156(2):155-66. doi: 10.1016/0378-1119(95)00020-7.

DOI:10.1016/0378-1119(95)00020-7

PMID:7758952

Abstract

In order to gain a better understanding of the development and evolution of cereal flowers, we have cloned and sequenced two MADS-box genes from maize and the partial cDNA of a third one. One of the genomic clones was identified as ZAG2 (Zea AGAMOUS 2), while the other has a very similar structure and the potential to encode a protein which shares 94% sequence identity with the putative ZAG2 gene product. The cDNA reveals considerable similarity to ZAG1. Phylogenetic evaluation of the sequence information, as well as chromosomal localization, both suggest that we have identified two pairs of AGAMOUS-like MADS-box genes which were created during duplication of chromosomal segments or complete chromosomes.

摘要

为了更好地理解谷类植物花的发育和进化，我们从玉米中克隆并测序了两个MADS盒基因以及第三个基因的部分cDNA。其中一个基因组克隆被鉴定为ZAG2（玉米AGAMOUS 2），而另一个具有非常相似的结构，并且有可能编码一种与推定的ZAG2基因产物具有94%序列同一性的蛋白质。该cDNA与ZAG1具有相当大的相似性。对序列信息的系统发育评估以及染色体定位均表明，我们已经鉴定出两对在染色体片段或完整染色体复制过程中产生的类AGAMOUS MADS盒基因。