一种韩国大花蕙兰花叶病毒分离株外壳蛋白编码cDNA的克隆与测序
Cloning and sequencing of a cDNA encoding the coat protein of a Korean isolate of cymbidium mosaic virus.
作者信息
Ryu K H, Yoon K E, Park W M
机构信息
Department of Agricultural Biology, College of Natural Resources, Korea University, Seoul.
出版信息
Gene. 1995 Apr 24;156(2):303-4. doi: 10.1016/0378-1119(95)00105-f.
The 3'-terminal nucleotide (nt) sequence of the coat protein (CP)-encoding gene of the Korean isolate of cymbidium mosaic potexvirus (CyMV-K) has been determined. The sequence contained a full-length ORF coding for the viral CP. It comprises 663 nt and a polypeptide chain of 220 amino acids with a M(r) of 23,718. The stop codon (TGA) is followed by a 3'-untranslated region (3'-UTR) of 79 nt and a poly(A) tract. The putative polyadenylation signal, AATAAA motif, is found in the terminus of the 3'-UTR.
已确定了大花蕙兰花叶病毒韩国分离株（CyMV-K）外壳蛋白（CP）编码基因的3'末端核苷酸（nt）序列。该序列包含一个编码病毒CP的全长开放阅读框（ORF）。它由663个nt组成，编码一条含有220个氨基酸的多肽链，分子量为23,718。终止密码子（TGA）之后是一个79 nt的3'非翻译区（3'-UTR）和一个聚腺苷酸尾。在3'-UTR末端发现了推定的聚腺苷酸化信号AATAAA基序。
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