Orfao A, Ruiz-Arguelles A, Lacombe F, Ault K, Basso G, Danova M
Laboratory of Hematology, University Hospital of Salamanca, Spain.
Haematologica. 1995 Jan-Feb;80(1):69-81.
The techniques of flow cytometry are becoming more and more important for the clinical hematology laboratory. No longer a novelty confined to a few specialized institutions as it was 10 years ago, flow cytometry has blossomed into a mature discipline. The methodology is well-known, the mechanical apparatus is readily available, and the role it plays in clinical hematology is increasingly appreciated. The burgeoning number of scientific articles devoted to this topic attests to the interest it has aroused as a tool for both medical research and patient care. In fact, more than a thousand such papers are now published each year and it would be impossible to deal with all the methodologies and applications of FCM currently utilized or under development. Throughout this paper four relevant hematologic fields are briefly discussed, in which FCM appears to be of great help at present: the immunophenotyping of leukemias and lymphomas, the measurement of proliferative activity and DNA ploidy in hematological malignancies, the detection of drug resistant leukemic cells and the use of FCM in the study of platelets.
流式细胞术技术对临床血液学实验室正变得越来越重要。不再像10年前那样只是局限于少数专业机构的新奇事物，流式细胞术已发展成为一门成熟的学科。其方法广为人知，机械设备 readily available，并且它在临床血液学中所起的作用越来越受到重视。致力于该主题的科学文章数量迅速增加，证明了它作为医学研究和患者护理工具所引起的兴趣。事实上，现在每年发表超过一千篇此类论文，要处理目前使用或正在开发的FCM的所有方法和应用是不可能的。在本文中，简要讨论了四个相关的血液学领域，目前FCM在这些领域似乎有很大帮助：白血病和淋巴瘤的免疫表型分析、血液系统恶性肿瘤中增殖活性和DNA倍体的测量、耐药白血病细胞的检测以及FCM在血小板研究中的应用。
