Anorexigenic effect of fecal extract from ventromedial hypothalamic (VMH) lesioned rats on normal rats.

Feces of ventromedial hypothalamus (VMH) lesioned obese and sham operated (SL) rats were separately pooled and subjected to column chromatography using sephadex G-150. Fractions showing peak absorption at 280 nm, obtained from respective group of rats were separately pooled and divided into 9 equal aliquots and injected ip in two groups of overnight fasted normal rats (OFN rats). Rats injected with fecal extract from VMH lesioned rats showed decreased food and water intake which was not seen in rats treated with the fecal extract from SL rats suggesting the possible presence of an anorexigenic substance in the feces of VMH lesioned rats but absent in SL rats.