Chen J C, Liu S M, Fei H B, Gong W L
Research Unit of Hematological Genetics, Xiehe Hospital, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1994;14(4):204-8. doi: 10.1007/BF02897668.
DNA from 36 patients with chronic myelogenous leukemia (CML) at various clinical stages and 6 cases of acute leukemia was investigated for alterations of the p53 gene by Southern blot analysis. Rearrangements of the p53 gene were seen in 3 of 12 (25.00%) cases of blast crisis and accelerated phase (AP) of CML and in only one of 18 chronic phrase (CP), just as has been reported previously. Meanwhile, by restriction fragment length polymorphism (RFLP) analysis the Bgl II site polymorphism in the p53 gene was also found. The frequency in Chinese people detected here was 0.392, which was strikingly higher than that in some other countries (P < 0.001). These results suggested that the alterations of the p53 gene, for example, p53 rearrangements, were probably responsible for the progression of BC in some CML patients, and that the frequency of Bgl II polymorphism in the p53 gene might be related to the population distribution.
采用Southern印迹分析法，对36例处于不同临床阶段的慢性粒细胞白血病（CML）患者及6例急性白血病患者的DNA进行p53基因改变情况的研究。正如之前所报道的，在12例慢性粒细胞白血病急变期和加速期（AP）患者中有3例（25.00%）发现了p53基因重排，而在18例慢性期（CP）患者中仅有1例出现重排。同时，通过限制性片段长度多态性（RFLP）分析，还发现了p53基因中的Bgl II位点多态性。此处检测的中国人中的频率为0.392，显著高于其他一些国家（P < 0.001）。这些结果表明，p53基因改变，如p53重排，可能是某些慢性粒细胞白血病患者急变期进展的原因，并且p53基因中Bgl II多态性的频率可能与人群分布有关。
