Sohma Y, Sasano H, Shiga R, Saeki S, Suzuki T, Nagura H, Nose M, Yamamoto T
Tohoku University Gene Research Center, Tohoku University, Sendai, Japan.
Proc Natl Acad Sci U S A. 1995 May 23;92(11):4937-41. doi: 10.1073/pnas.92.11.4937.
By screening a cDNA library constructed from aortic total RNA derived from Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the kappa light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin gamma heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.
通过差异杂交筛选从遗传性高脂血症（WHHL）兔主动脉总RNA构建的cDNA文库，我们获得了一个编码免疫球蛋白κ轻链的cDNA。对不同年龄的WHHL兔和正常兔主动脉制备的总RNA进行Northern印迹分析，结果显示，在WHHL兔的主动脉中，这种轻链mRNA随年龄逐渐积累。用兔免疫球蛋白γ重链mRNA特异性反义寡核苷酸进行Northern印迹和原位杂交，也检测到这种重链mRNA在WHHL兔主动脉晚期病变中的积累。此外，免疫组织化学和电子显微镜分析表明，动脉粥样硬化病变中存在浆细胞。
