静电和疏水相互作用对含铝佐剂吸附蛋白质的贡献。

Contribution of electrostatic and hydrophobic interactions to the adsorption of proteins by aluminium-containing adjuvants.

作者信息

al-Shakhshir R H, Regnier F E, White J L, Hem S L

机构信息

Department of Industrial and Physical Pharmacy, Pudue University, West Lafayette, IN 47907, USA.

出版信息

Vaccine. 1995 Jan;13(1):41-4. doi: 10.1016/0264-410x(95)80009-3.

DOI:10.1016/0264-410x(95)80009-3

PMID:7762276

Abstract

The effect of ionic strength and ethylene glycol on the adsorption of bovine serum albumin (BSA) or lysozyme by a commercial aluminium hydroxide or aluminium phosphate adjuvant was studied at pH 7.4 and 25 degrees C. The adsorption of BSA by aluminium hydroxide adjuvant and lysozyme by aluminium phosphate adjuvant was found to be inversely related to ionic strength. This indicates that electrostatic attractive forces contribute to adsorption. The adsorption of lysozyme by aluminium phosphate adjuvant was reduced by the addition of ethylene glycol. However, no change in the adsorption of BSA by aluminium hydroxide adjuvant was noted when up to 40% ethylene glycol was present. This behaviour indicates that hydrophobic forces contribute to the adsorption of lysozyme but not of BSA. However, virtually no adsorption was observed when the protein and the adjuvant had the same surface charge. Thus, attractive forces may not be sufficient to produce adsorption of an antigen by an aluminium-containing adjuvant if electrostatic repulsive forces are present.

摘要

在pH 7.4和25摄氏度条件下，研究了离子强度和乙二醇对市售氢氧化铝或磷酸铝佐剂吸附牛血清白蛋白（BSA）或溶菌酶的影响。发现氢氧化铝佐剂对BSA的吸附以及磷酸铝佐剂对溶菌酶的吸附与离子强度呈负相关。这表明静电吸引力有助于吸附。添加乙二醇会降低磷酸铝佐剂对溶菌酶的吸附。然而，当存在高达40%的乙二醇时，未观察到氢氧化铝佐剂对BSA吸附的变化。这种行为表明疏水力有助于溶菌酶的吸附，但对BSA的吸附没有作用。然而，当蛋白质和佐剂具有相同的表面电荷时，几乎没有观察到吸附现象。因此，如果存在静电排斥力，吸引力可能不足以使含铝佐剂吸附抗原。