Gagos S, Hopwood V L, Iliopoulos D, Kostakis A, Karayannakos P, Yatzides H, Skalkeas G D, Pathak S
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Anticancer Res. 1995 Mar-Apr;15(2):369-78.
The purpose of this study was to identify specific chromosomal abnormalities that might be involved in colon cancer metastasis. For this reason, we performed extensive karyotypic analysis on two colon cancer cell lines (SW480 and SW620) established from two surgical biopsies taken at different intervals and representing different stages of the disease from the same patient. Despite the karyotypic heterogeneity, several marker chromosomes were shared between the two cell lines, indicating their common origin. We hypothesized that these shared chromosomal aberrations might be critical for the continuous growth of the tumor cells and, therefore, were retained through progression of the disease. Duplication of 16q and new or additional structural chromosomal abnormalities involving breakpoints 3p21, 8p11, 10q25, 13q14, 14q11 and 15q15 were observed as the characteristic anomalies only in the SW620 cell line. As SW620 was established from the abdominal metastatic lesion of the patient, we postulated that the acquisition of these new markers in the progression steps of the primary tumor might represent "hot-spots" that possibly contain genes crucial for metastatic potential in colon cancer.
本研究的目的是确定可能与结肠癌转移有关的特定染色体异常。因此，我们对两个结肠癌细胞系（SW480和SW620）进行了广泛的核型分析，这两个细胞系来自同一患者在不同时间间隔采集的两份手术活检样本，代表了疾病的不同阶段。尽管核型存在异质性，但两个细胞系之间共有几条标记染色体，表明它们有共同的起源。我们假设这些共有的染色体畸变可能对肿瘤细胞的持续生长至关重要，因此在疾病进展过程中得以保留。仅在SW620细胞系中观察到16q重复以及涉及3p21、8p11、10q25、13q14、14q11和15q15断点的新的或额外的结构染色体异常，这些被视为特征性异常。由于SW620是从患者的腹部转移病灶建立的，我们推测在原发性肿瘤进展过程中获得这些新标记可能代表“热点”，其中可能包含对结肠癌转移潜能至关重要的基因。
