Carlsson G, Larsson P A, Frösing R, Hafström L O, Spears C P, Peterson A, Gustavsson B
Department of Surgery, Ostra sjukhuset, Göteborg, Sweden.
Anticancer Res. 1995 Mar-Apr;15(2):433-9.
Transplantable nitrosoguanidine-induced rat colonic carcinoma was transplanted into the liver of 49 rats in six different series during three years. Tumor growth in vivo was surveyed by repeated laparotomy at day eight and day seventeen, and 25 rats were treated with 5-fluorouracil (30 mg/kg body weight) once a day, between day 8 and day 17. Tumor take was one hundred per cent and the tumor growth rate was similar throughout the experimental period. No animals died due to treatment or due to progressive tumor growth. The animals treated with 5-fluorouracil revealed a 5 per cent weight loss compared to the controls but no other signs of health deterioration were observed. The tumors treated with 5-fluorouracil had a 70 per cent decrease of growth rate compared to control rats. Exposure of the tumor cells in vitro to 5-fluorouracil induced a dose--related decrease in surviving cells with a 50 per cent reduction of surviving cells 48 hours after exposure to 0.01 mg/ml of 5-fluorouracil. Thus, we present here a new, feasible and reproducible animal model, excellently suited to in vivo and in vitro studies of fluorinated pyrimidines and solid tumor growth.
在三年期间，将可移植的亚硝基胍诱导的大鼠结肠癌移植到六个不同系列的49只大鼠的肝脏中。在第8天和第17天通过重复剖腹术对体内肿瘤生长进行监测，并且在第8天至第17天之间，25只大鼠每天接受一次5-氟尿嘧啶（30mg/kg体重）治疗。肿瘤接种成功率为100%，并且在整个实验期间肿瘤生长速率相似。没有动物因治疗或肿瘤进展而死亡。与对照组相比，接受5-氟尿嘧啶治疗的动物体重减轻了5%，但未观察到其他健康恶化迹象。与对照大鼠相比，用5-氟尿嘧啶治疗的肿瘤生长速率降低了70%。体外将肿瘤细胞暴露于5-氟尿嘧啶会导致存活细胞数量呈剂量相关减少，在暴露于0.01mg/ml的5-氟尿嘧啶48小时后，存活细胞减少50%。因此，我们在此提出一种新的、可行且可重复的动物模型，非常适合对氟嘧啶和实体瘤生长进行体内和体外研究。
