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嗜热栖热菌HB8的3-异丙基苹果酸脱氢酶的组氨酸273参与辅酶结合。

His273 of 3-isopropylmalate dehydrogenase from Thermus thermophilus HB8 is involved in the coenzyme binding.

作者信息

Yaoi T, Miyazaki K, Oshima T

机构信息

Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Biochem Biophys Res Commun. 1995 May 25;210(3):733-7. doi: 10.1006/bbrc.1995.1720.

DOI:10.1006/bbrc.1995.1720
PMID:7763246
Abstract

The coenzyme binding site of NAD(+)-dependent 3-isopropylmalate dehydrogenase from Thermus thermophilus was analyzed by chemical modification and site-directed mutagenesis, and His273 of the enzyme was identified to be involved in the coenzyme binding.

摘要

通过化学修饰和定点诱变分析了嗜热栖热菌中依赖烟酰胺腺嘌呤二核苷酸（NAD⁺）的3-异丙基苹果酸脱氢酶的辅酶结合位点，该酶的组氨酸273被确定参与辅酶结合。

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