Bustamante C, Gurrieri S, Smith S B
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Trends Biotechnol. 1993 Jan;11(1):23-30. doi: 10.1016/0167-7799(93)90071-G.
An improved understanding of the molecular processes of migration and reorientation of DNA molecules undergoing conventional and pulsed-field gel electrophoresis (PFGE) should provide the basis for the rational optimization of these techniques. This review of common pulsed-field methods emphasizes the range of their applications as well as their current limitations. The molecular mechanisms involved in electrophoresis, as they are beginning to emerge from a combination of direct observations using a microscope and computer-modeling studies, are also discussed.
