Yamane T
Department of Food Science and Technology, School of Agriculture, Nagoya University, Japan.
Biotechnol Prog. 1993 Jan-Feb;9(1):81-5. doi: 10.1021/bp00019a012.
Recent advances in the technology of continuous in situ turbidity measurements in microbial cultivations are reviewed. Some characteristics of commercially available on-line laser turbidimeters are estimated in relation to requirements of sensors for microbial cultures. A system for the automation of fed-batch cultures using an on-line turbidimeter is proposed. Experimental data are presented concerning fed-batch cultures grown on synthetic and natural complex media. In situ turbidity data were found to be particularly useful for the automation of the feeding of natural complex nutrient solutions by converting the data into dry cell mass concentration and at the same time utilizing the instantaneous data on the culture broth volume through a computer.
综述了微生物培养中连续原位浊度测量技术的最新进展。根据微生物培养传感器的要求，评估了市售在线激光浊度仪的一些特性。提出了一种使用在线浊度仪实现分批补料培养自动化的系统。给出了在合成培养基和天然复合培养基上进行分批补料培养的实验数据。通过计算机将原位浊度数据转换为干细胞质量浓度，并同时利用培养液体积的瞬时数据，发现原位浊度数据对于天然复合营养液补料的自动化特别有用。
