Zigangirova N A, Popova O V, Solovjeva C V, Gintzburg A L, Prozorovsky S V
Laboratory of L-form and Mycoplasmas, Gamaleya Institute, Moscow, Russia.
Lett Appl Microbiol. 1993 Feb;16(2):106-9. doi: 10.1111/j.1472-765x.1993.tb00357.x.
The polymerase chain reaction was used to detect clinical samples of Mycoplasma pneumoniae. A 245-bp region of the cytoadhesin P1 gene was shown to be specifically amplified in Myc. pneumoniae, but not in other species of Mollicutes. Picogram amounts of Myc. pneumoniae. DNA could be detected per ml blood serum by use of a simple and reliable protocol for sample preparation and a PCR reaction involving two rounds of amplification. Application of the PCR-based method for the detection of Myc. pneumoniae in serum samples and throat swabs from patients with atypical pneumonia showed that it could be used in clinical diagnosis.
采用聚合酶链反应检测肺炎支原体临床样本。结果显示，细胞黏附素P1基因的一个245bp区域在肺炎支原体中能被特异性扩增，而在其他支原体属物种中则不能。通过使用一种简单可靠的样本制备方案和两轮扩增的聚合酶链反应，每毫升血清中皮克量的肺炎支原体DNA即可被检测到。将基于聚合酶链反应的方法应用于非典型肺炎患者血清样本和咽拭子中肺炎支原体的检测，结果表明该方法可用于临床诊断。
