通过酿酒酵母的补料分批培养生产重组蛋白。

Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae.

作者信息

Hardjito L, Greenfield P F, Lee P L

机构信息

Chemical Engineering Department, University of Queensland, St. Lucia, Australia.

出版信息

Enzyme Microb Technol. 1993 Feb;15(2):120-6. doi: 10.1016/0141-0229(93)90035-z.

DOI:10.1016/0141-0229(93)90035-z

PMID:7763453

Abstract

Protein production with the recombinant yeast Saccharomyces cerevisiae in fed-batch culture is investigated in this work using beta-galactosidase as a model protein. Segregational instability was negligible during the observed culture periods. The final volumetric productivity, as determined by both cell concentration and gene expression, was strongly affected by the time course of the glucose levels in the bioreactor. It was found that an average glucose feed rate of 1.31 g glucose h-1 resulted in both the maximum beta-galactosidase production rate of 831-950 units ml-1 h-1 and the maximum cell production rate of 0.520-0.524 mg ml-1 h-1.

摘要

本研究以β-半乳糖苷酶作为模型蛋白，对重组酵母酿酒酵母在补料分批培养中的蛋白质生产进行了研究。在观察到的培养期间，分离不稳定性可忽略不计。由细胞浓度和基因表达所确定的最终体积生产力，受到生物反应器中葡萄糖水平随时间变化过程的强烈影响。结果发现，平均葡萄糖进料速率为1.31 g葡萄糖 h-1时，β-半乳糖苷酶的最大生产率可达831 - 950单位 ml-1 h-1，细胞的最大生产率可达0.520 - 0.524 mg ml-1 h-1。