Wedrychowski A, Kim Y W, Chang T W
Tanox Biosystems, Inc., Houston, TX 77025.
Biotechnology (N Y). 1993 Apr;11(4):486-9. doi: 10.1038/nbt0493-486.
Anti-CD3 antibodies of some IgG subclasses are very potent T lymphocyte mitogens in vitro and, seemingly contradictorily, very effective immunosuppressive agents in vivo. Using hamster anti-murine CD3 monoclonal antibody, 2C11, as a model, we have found that 2C11.IgG, or its F(ab')2 fragment, coupled to microbeads can provide short-term and vigorous activation of T cells and expansion of the lymphoid system in vivo. In contrast to free 2C11.IgG, these conjugates do not kill mice and cause T cell depletion, and can enhance immune responses. This study suggests that properly modified anti-CD3 antibodies can serve as in vivo immune system enhancers potentially useful in the treatment of cancer and chronic infectious diseases.
某些IgG亚类的抗CD3抗体在体外是非常有效的T淋巴细胞有丝分裂原，而在体内似乎矛盾地是非常有效的免疫抑制剂。以仓鼠抗小鼠CD3单克隆抗体2C11为模型，我们发现与微珠偶联的2C11.IgG或其F(ab')2片段可在体内提供T细胞的短期强力激活和淋巴系统的扩张。与游离的2C11.IgG相反，这些偶联物不会杀死小鼠和导致T细胞耗竭，反而可增强免疫反应。本研究表明，经过适当修饰的抗CD3抗体可作为体内免疫系统增强剂，可能对癌症和慢性传染病的治疗有用。
