Lyutzkanova D, Nikolova B, Stoilova-Discheva M, Todorov T
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Lett Appl Microbiol. 1993 Apr;16(4):217-9. doi: 10.1111/j.1472-765x.1993.tb01401.x.
Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature.
从在含有2%甘氨酸的两阶段培养系统中生长至对数后期的菌丝体中分离出再生频率最高的黄色链霉菌原生质体。在测试的11种再生培养基中,R9被选为最有效的培养基,29℃为最佳温度。