Bryjak J, Noworyta A
Institute of Chemical Engineering, Technical University of Wrocław, Poland.
J Chem Technol Biotechnol. 1993;57(1):79-85. doi: 10.1002/jctb.280570114.
The effects of glutaraldehyde, enzyme concentrations and reactants volumes, ionic strength, pH value and carrier particle diameter on immobilization of penicillin acylase onto acrylic carriers were studied. The activity of immobilized enzyme preparations was also studied over a range of pH values and temperatures and thermal and pH stabilities were determined. The use of the immobilized preparation for penicillin G hydrolysis in a batch reactor was investigated. The immobilized enzyme gave a significant reduction in hydrolysis time compared to hydrolysis by the native enzyme.
研究了戊二醛、酶浓度、反应物体积、离子强度、pH值和载体粒径对青霉素酰化酶固定到丙烯酸载体上的影响。还研究了固定化酶制剂在一系列pH值和温度范围内的活性，并测定了其热稳定性和pH稳定性。研究了固定化制剂在间歇反应器中用于青霉素G水解的情况。与天然酶水解相比，固定化酶显著缩短了水解时间。
