固定化抗体对抗抗原或固定化抗原对抗抗体的结合动力学：侧向相互作用和可变速率系数的影响

Binding kinetics of antigen by immobilized antibody or of antibody by immobilized antigen: influence of lateral interactions and variable rate coefficients.

作者信息

Sadana A, Madugula A

机构信息

Chemical Engineering Department, University of Mississippi, University 38677-9740.

出版信息

Biotechnol Prog. 1993 May-Jun;9(3):259-66. doi: 10.1021/bp00021a004.

DOI:10.1021/bp00021a004

PMID:7763695

Abstract

Kinetic rate expressions are developed for the binding kinetics of antigen by immobilized antibody or of antibody by immobilized antigen. The influence of lateral interactions leads to variable rate coefficients for adsorption, and these provide a more realistic picture of the events occurring on the surface. Emphasis is placed on the similarities and dissimilarities present in the kinetic rate expressions. A general trend in the rate expressions is observed on going from dual-step binding to dual-step binding with lateral interactions. This provides physical insights into the reactions occurring at the surface and helps in their control and stability at the surface.

摘要

针对固定化抗体对抗抗原或固定化抗原对抗抗体的结合动力学，推导了动力学速率表达式。横向相互作用的影响导致吸附的速率系数可变，这些系数为表面发生的事件提供了更真实的描述。重点在于动力学速率表达式中存在的异同点。从双步结合到具有横向相互作用的双步结合，在速率表达式中观察到一种普遍趋势。这为表面发生的反应提供了物理见解，并有助于控制其在表面的反应及其稳定性。