Huang N, Durica D S
Department of Zoology, University of Oklahoma, Norman 73019.
SAAS Bull Biochem Biotechnol. 1993;6:21-30.
A commonly used RNA isolation technique that employs a single-step acid phenol-chloroform extraction (Chomczynski and Sacchi, 1987) produces a different distribution pattern of C. erythrocephala c-abl (Ceabl) transcripts following Northern blot analysis than methods that rely on phenol-chloroform extraction at basic pH (Jowett, 1986). Only Ceabl transcripts of 10.5 kb and 6.5 kb are recovered, while Ceabl transcripts of 9 kb and 4.4 kb are lost, when acidic phenol-chloroform methods are used. All tested extraction methods which do not rely on phenol-chloroform extraction under acidic conditions can recover Ceabl transcripts of 10.5 kb, 9 kb, 6.5 kb and 4.4 kb. The reason for this discrepancy is apparently due to differential loss of mRNA at the phenol phase and/or interphase boundary during the acidic extractions.
一种常用的RNA分离技术采用单步酸性酚-氯仿提取法（乔姆钦斯基和萨基，1987年），在Northern印迹分析后，与依赖碱性pH值下酚-氯仿提取的方法相比，产生了不同的红斑头蝇c-abl（Ceabl）转录本分布模式（乔伊特，1986年）。当使用酸性酚-氯仿方法时，仅回收10.5 kb和6.5 kb的Ceabl转录本，而9 kb和4.4 kb的Ceabl转录本则丢失。所有不依赖酸性条件下酚-氯仿提取的测试提取方法都能回收10.5 kb、9 kb、6.5 kb和4.4 kb的Ceabl转录本。这种差异的原因显然是由于酸性提取过程中mRNA在酚相和/或相间边界处的不同程度损失。
