Satoh M, Takeuchi M
Res Commun Inst Ferment. 1993(16):6-17.
Hypoxanthine/guanine phosphoribosyl transferase (HPRT)-deficient cell lines, designated as Neuro-2aTG and PC12TG, were established from mouse neuroblastoma Neuro-2a and rat pheochromocytoma PC12, respectively. Both cell lines stably exhibited HPRT- phenotype, and expressed neuronal properties, i.e., constitutive expression of 200-kD neurofilament protein in Neuro-2aTG and responsiveness to NGF in PC12TG. Therefore, these cell lines will be useful as fusion partners in somatic cell hybridization with neurons.
次黄嘌呤/鸟嘌呤磷酸核糖转移酶（HPRT）缺陷细胞系，分别命名为Neuro-2aTG和PC12TG，是从小鼠神经母细胞瘤Neuro-2a和大鼠嗜铬细胞瘤PC12建立的。这两种细胞系均稳定表现出HPRT-表型，并表达神经元特性，即Neuro-2aTG中200-kD神经丝蛋白的组成性表达以及PC12TG中对神经生长因子（NGF）的反应性。因此，这些细胞系将作为与神经元进行体细胞杂交的融合伙伴。
