Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7.
作者信息
Tsujibo H, Orikoshi H, Imada C, Okami Y, Miyamoto K, Inamori Y
机构信息
Osaka University of Pharmaceutical Sciences, Japan.
出版信息
Biosci Biotechnol Biochem. 1993 Aug;57(8):1396-7. doi: 10.1271/bbb.57.1396.
PMID:7764022
Abstract
Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.
摘要
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