Pires M J, Cabral J M
Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Lisboa, Portugal.
Biotechnol Prog. 1993 Nov-Dec;9(6):647-50. doi: 10.1021/bp00024a012.
Recombinant cytochrome b5 was extracted to a reversed micelle phase of a cationic surfactant (hexadecyltrimethylammonium bromide, CTAB) in cyclohexane/decanol and back-extracted to a fresh aqueous phase. Depending on the experimental conditions, i.e., temperature, pH, and ionic strength, the extraction is controlled by either hydrophobic (pH close to the pI and high ionic strength) or electrostatic (pH far from the pI and low ionic strength) interactions. The protein interacts strongly with the surfactant aggregate once it is extracted to the organic phase, and it is very difficult to back-extract it to a new aqueous phase.
重组细胞色素b5被萃取到环己烷/癸醇中阳离子表面活性剂（十六烷基三甲基溴化铵，CTAB）的反胶束相中，然后再反萃取到新鲜水相中。根据实验条件，即温度、pH值和离子强度，萃取过程由疏水作用（pH值接近蛋白质的等电点且离子强度高）或静电作用（pH值远离蛋白质的等电点且离子强度低）控制。蛋白质一旦被萃取到有机相中，就会与表面活性剂聚集体强烈相互作用，很难再反萃取到新的水相中。
