含α-L-吡喃甘露糖基二糖和酚类的合成物作为市售柚苷酶α-L-甘露糖苷酶活性的底物
Synthesis of alpha-L-mannopyranosyl-containing disaccharides and phenols as substrates for the alpha-L-mannosidase activity of commercial naringinase.
作者信息
Esaki S, Ohishi A, Katsumata A, Sugiyama N, Kamiya S
机构信息
Department of Food Science, School of Food and Nutritional Sciences, University of Shizuoka, Japan.
出版信息
Biosci Biotechnol Biochem. 1993 Dec;57(12):2099-103. doi: 10.1271/bbb.57.2099.
In order to clarify the substrate specificity of the alpha-L-mannosidase activity of naringinase (Sigma), the following disaccharides and phenol glycosides were freshly prepared: methyl 2-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (1), methyl 3-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (2), methyl 4-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (3), methyl 5-O-(alpha-L-mannopyranosyl)-beta-D-glucoside (4), methyl 6-O-(alpha-L-mannopyranosyl)-alpha-D-glucoside (5), 6-O-(alpha-L-mannopyranosyl)-D-galactose (6), p-nitrophenyl alpha-L-mannoside (7), and 4-methyl umbelliferone alpha-L-mannoside (8). These compounds, except for 3 and 5 were hydrolyzed with naringinase.
为了阐明柚苷酶（Sigma）的α-L-甘露糖苷酶活性的底物特异性，新制备了以下二糖和酚糖苷：2-O-（α-L-吡喃甘露糖基）-β-D-葡萄糖苷甲酯（1）、3-O-（α-L-吡喃甘露糖基）-α-D-葡萄糖苷甲酯（2）、4-O-（α-L-吡喃甘露糖基）-α-D-葡萄糖苷甲酯（3）、5-O-（α-L-吡喃甘露糖基）-β-D-葡萄糖苷甲酯（4）、6-O-（α-L-吡喃甘露糖基）-α-D-葡萄糖苷甲酯（5）、6-O-（α-L-吡喃甘露糖基）-D-半乳糖（6）、对硝基苯基α-L-甘露糖苷（7）和4-甲基伞形酮α-L-甘露糖苷（8）。除3和5外，这些化合物均被柚苷酶水解。
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