Fujii M, Matsumura N, Mito K, Shimizu T, Kuwahara M, Sugano S, Karaki H
Foods Division, Asahi Chemical Industry Co., Ltd., Shizuoka, Japan.
Biosci Biotechnol Biochem. 1993 Dec;57(12):2186-8. doi: 10.1271/bbb.57.2186.
An autolysate of bonito bowels was treated with ultrafiltration, loose RO concentration, ion-exchange chromatography, and reverse phase chromatography to increase its potency to inhibit angiotensin I-converting enzyme (ACE) activity by 16-fold. Oral administration of the partially purified autolysate decreased the systolic blood pressure of spontaneously hypertensive rats (SHR) in a dose-dependent manner at the doses of 1 g peptides/kg or higher. The relationship between the antihypertensive activity (in vivo) of the partially purified preparation and its ACE inhibitory activity (in vitro) in comparison with previously reported ACE inhibitory peptides is discussed.
对鲣鱼肠道的自溶产物进行超滤、疏松反渗透浓缩、离子交换色谱和反相色谱处理，以将其抑制血管紧张素I转换酶（ACE）活性的效力提高16倍。口服部分纯化的自溶产物，在剂量为1 g肽/千克或更高时，以剂量依赖的方式降低了自发性高血压大鼠（SHR）的收缩压。讨论了部分纯化制剂的降压活性（体内）与其ACE抑制活性（体外）之间的关系，并与先前报道的ACE抑制肽进行了比较。
