胆汁酸的酶分析
Enzymic analysis of bile acids.
作者信息
MacDonald I A
出版信息
Clin Biochem. 1976 Jun;9(3):153-4. doi: 10.1016/s0009-9120(76)80039-2.
PMID:776443
Abstract
- The use of enzymes and thin-layer chromatography (TLC) in quantifying individual bile acids from bile or serum is reviewed. 2. Both 3alpha-hydroxysteroid dehydrogenase (commercially available P. testosteroni enzyme) and 7alpha-hydroxysteroid dehydrogenase (E. coli) can be used for spectrophotometric or fluorometric measurement of individual bile acids in TLC eluates and to estimate pool size and half-life of serially labelled samples.
摘要
- 本文综述了酶和薄层色谱法（TLC）在定量分析胆汁或血清中各胆汁酸方面的应用。2. 3α-羟基类固醇脱氢酶（市售的睾丸酮假单胞菌酶）和7α-羟基类固醇脱氢酶（大肠杆菌）均可用于对TLC洗脱液中的各胆汁酸进行分光光度法或荧光法测量，并估算连续标记样品的池大小和半衰期。
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