Middendorf C, Schulze B, Freitag R, Scheper T, Howaldt M, Hoffmann H
Abteilung Biotechnologie, Westfälische Wilhelms-Universität Münster, Germany.
J Biotechnol. 1993 Dec;31(3):395-403. doi: 10.1016/0168-1656(93)90083-y.
Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalysis.
诸如酶联免疫吸附测定（ELISA）之类的免疫分析技术常被用于检测培养过程中产生的蛋白质。由于耗时的传统ELISA自动化困难，对合适的在线监测方法有强烈需求。将知名免疫测定法与流动注射分析（FIA）技术相结合，我们提出了非均相免疫FIA法和比浊免疫FIA法。用这些FIA方法研究了以下蛋白质：耐热支链淀粉酶、免疫球蛋白G（IgG）、抗凝血酶III和重组组织型纤溶酶原激活剂。在支链淀粉酶和小鼠单克隆IgG的情况下，比浊免疫FIA用于培养过程的在线分析。此处展示的结果证明了这些免疫分析的有效性和应用。
