Peberdy J F
Department of Life Science, University of Nottingham, UK.
Trends Biotechnol. 1994 Feb;12(2):50-7. doi: 10.1016/0167-7799(94)90100-7.
Protein secretion is important in all fungi. The majority of proteins by fungi are thought to be glycosylated and many of them are structurally associated with the cell envelope, the plasma membrane and the cell wall. Many of the enzymes secreted by fungi have been incorporated into commercial processes and used in a range of industries. The existence of strains producing very high levels of secreted enzymes stimulated interest in the use of fungi as hosts for the expression of recombinant proteins. Despite the attention that protein secretion in fungi has attracted, and the multifaceted importance of the process, our understanding of the cellular mechanisms involved is still minimal and, for the most part, it is necessary to extrapolate from other eukaryotic organisms. However, current research suggests that protein secretion in filamentous fungi is intimately associated with the process of growth at the hyphal tip. Such unique features merit a detailed study of this important phenomenon.
蛋白质分泌在所有真菌中都很重要。真菌产生的大多数蛋白质被认为是糖基化的，其中许多在结构上与细胞膜、质膜和细胞壁相关联。真菌分泌的许多酶已被应用于商业生产过程，并在一系列行业中得到使用。能够产生非常高水平分泌酶的菌株的存在，激发了人们将真菌用作重组蛋白表达宿主的兴趣。尽管真菌中的蛋白质分泌已引起广泛关注，且该过程具有多方面的重要性，但我们对其中涉及的细胞机制的了解仍然很少，在很大程度上，有必要从其他真核生物中进行推断。然而，目前的研究表明，丝状真菌中的蛋白质分泌与菌丝尖端的生长过程密切相关。这些独特的特征值得对这一重要现象进行详细研究。
