Ohnishi H, Nikaidou N, Kamio Y, Izaki K
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 1994 Feb;58(2):432-3. doi: 10.1271/bbb.58.432.
A pectin lyase defective mutant was constructed from Erwinia carotovora Er by transposon Tn5 insertion mutagenesis to analyze the promoter region of the pnl gene, which had been cloned. The promoter of pnl is between -140 and -74 upstream of the structural gene of pnl and appears not to be regulated by Lex A.