重组大肠杆菌TG1在合成培养基上生产人甲状旁腺激素。

Production of human parathyroid hormone by recombinant Escherichia coli TG1 on synthetic medium.

作者信息

Harder M P, Sanders E A, Wingender E, Deckwer W D

机构信息

GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.

出版信息

J Biotechnol. 1994 Feb 14;32(2):157-64. doi: 10.1016/0168-1656(94)90178-3.

DOI:10.1016/0168-1656(94)90178-3

PMID:7764562

Abstract

Production of human parathyroid hormone (hPTH) by Escherichia coli TG1:I52cIts was studied. The hPTH is expressed as a fusion protein under control of the bacteriophage lambda pR promoter. The organism grows on glucose/mineral salt medium and the expression of the gene product was investigated under variation of temperature and growth rate prior to and after induction. hPTH formation largely depends on cultivation temperature and is optimal for a temperature shift from 30 to 38 degrees C. Product expression is growth coupled and specific hPTH concentration is independent of growth rate. The results are compared with a previous study on E. coli N4830:pEX-PPTH grown on complex media.

摘要

对大肠杆菌TG1:I52cIts产生人甲状旁腺激素（hPTH）进行了研究。hPTH在噬菌体λ pR启动子的控制下作为融合蛋白表达。该菌株在葡萄糖/无机盐培养基上生长，并在诱导前后温度和生长速率变化的情况下研究基因产物的表达。hPTH的形成在很大程度上取决于培养温度，在温度从30℃转变到38℃时最适宜。产物表达与生长相关联，特定的hPTH浓度与生长速率无关。将这些结果与之前关于在复合培养基上生长的大肠杆菌N4830:pEX-PPTH的研究进行了比较。