Blasey H D, Bernard A R
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Cytotechnology. 1993;13(1):51-3. doi: 10.1007/BF00749975.
At the end of a hybridoma batch culture, the cells are usually discarded after separation from the culture broth. If, however, they are aseptically recycled into the reactor, the production process can be resumed simply by the addition of fresh medium. This cycle can then be repeated several times consecutively. In a test case, with a mouse hybridoma, we found antibody yields for each cycle in the same range as for a standard batch. In a 15 1 stirred tank reactor we could, within 6 days, produce 2.8 g of monoclonal antibody (MAb). This type of reactor operation allowed a doubling in the reactor volumetric productivity (mg/l/day).
在杂交瘤分批培养结束时，细胞通常在与培养液分离后被丢弃。然而，如果将它们无菌回收到反应器中，只需添加新鲜培养基就可以恢复生产过程。然后这个循环可以连续重复几次。在一个使用小鼠杂交瘤的测试案例中，我们发现每个循环的抗体产量与标准批次相同。在一个15升的搅拌罐反应器中，我们能够在6天内生产2.8克单克隆抗体（MAb）。这种反应器操作使反应器体积生产率（毫克/升/天）提高了一倍。
