Prior C P, Doyle K R, Duffy S A, Hope J A, Moellering B J, Prior G M, Scott R W, Tolbert W R
J Parenter Sci Technol. 1989 Jan-Feb;43(1):15-23.
The impact of continuous perfusion cell culture technology on production of one and two chain human rtPA, as well as monoclonal IgG and IgM, will be reviewed. Perfusion as compared to batch systems improve the quality of the product in the conditioned media in terms of biological activity and structural integrity. This significantly increases downstream recovery and daily production output of final purified material. These findings are a consequence of the ability of perfusion technology to 1) maintain cells intact at high cell density; 2) effectively reduce serum content to approximate serum free conditions; and 3) minimize residency time of labile product within the 37 degrees C environment of the bioreactor.
本文将综述连续灌注细胞培养技术对单链和双链人重组组织型纤溶酶原激活剂（rtPA）以及单克隆IgG和IgM生产的影响。与分批培养系统相比，灌注培养在生物活性和结构完整性方面提高了条件培养基中产品的质量。这显著提高了下游回收效率和最终纯化材料的日产量。这些发现是由于灌注技术能够：1）在高细胞密度下保持细胞完整；2）有效降低血清含量至接近无血清条件；3）将不稳定产品在生物反应器37摄氏度环境中的停留时间降至最低。
