Reddy S, Miller W M
Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208-3120.
Biotechnol Prog. 1994 Mar-Apr;10(2):165-73. doi: 10.1021/bp00026a006.
AB2-143.2 cells exhibit non-growth-associated antibody production (constant specific antibody production rate (qAb) throughout batch culture), while IND1 cells exhibit growth-associated production (decrease in qAb during stationary phase). AB2-143.2 cells increase qAb following abrupt batch osmotic stress. Enhanced antibody production in IND1 cells after batch osmotic shock is evidenced as maintenance of the higher exponential phase qAb into the stationary phase. AB2-143.2 cells also increase qAb in response to gradual osmotic stress in continuous culture. In contrast, IND1 cells decrease qAb during gradual osmotic stress. Although the two cell lines differ in antibody production, they have very similar intracellular antibody content profiles. Both show (1) constant antibody content during the exponential phase in control culture with a decrease as cells enter stationary phase; (2) maintenance of exponential-phase antibody content into the stationary phase after batch osmotic shock; and (3) no change in antibody content in response to gradual osmotic stress.
AB2 - 143.2细胞表现出与生长无关的抗体产生（在整个分批培养过程中特异性抗体产生速率恒定（qAb）），而IND1细胞表现出与生长相关的产生（在稳定期qAb降低）。AB2 - 143.2细胞在分批渗透压突然变化后qAb增加。分批渗透压冲击后IND1细胞中抗体产生增强表现为在稳定期维持较高的指数期qAb。AB2 - 143.2细胞在连续培养中对逐渐的渗透压变化也会增加qAb。相比之下，IND1细胞在逐渐的渗透压变化期间qAb降低。尽管这两种细胞系在抗体产生方面存在差异，但它们具有非常相似的细胞内抗体含量谱。两者都显示：（1）在对照培养的指数期抗体含量恒定，随着细胞进入稳定期而降低；（2）分批渗透压冲击后在稳定期维持指数期抗体含量；（3）对逐渐的渗透压变化抗体含量无变化。
