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具有人工信号序列的人生长激素的加工与分泌

Processing and secretion of human growth hormone with an artificial signal sequence.

作者信息

Kohara A, Yamamoto Y, Kikuchi M

机构信息

Protein Engineering Research Institute, Osaka, Japan.

出版信息

Biosci Biotechnol Biochem. 1994 Apr;58(4):779-81. doi: 10.1271/bbb.58.779.

DOI:10.1271/bbb.58.779
PMID:7764869
Abstract

We examined the secretion of human growth hormone in yeast cells with the artificial signal sequence L8LP, which is functional for human lysozyme secretion. The precursor was cleaved efficiently and the mature protein was secreted into the periplasmic space, but the protein aggregated. These results suggest that L8LP is also functional for human growth hormone. pI precipitation might be responsible for the aggregation.

摘要

我们用对人溶菌酶分泌具有功能的人工信号序列L8LP检测了酵母细胞中人生长激素的分泌情况。前体被有效切割，成熟蛋白分泌到周质空间，但该蛋白发生了聚集。这些结果表明，L8LP对人生长激素也具有功能。pI沉淀可能是聚集的原因。

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