Kurokawa M, Tominaga H, Ashida H, Sawa Y, Ochiai H
Laboratory of Applied Biological Science, Faculty of Agriculture, Shimane University, Japan.
Biosci Biotechnol Biochem. 1994 Apr;58(4):796-7. doi: 10.1271/bbb.58.796.
Single-stranded plasmid DNA of pPF1 from Phormidium foveolarum that was specifically degraded by S1 nuclease was detected by Southern hybridization. This is also the case of the homologous plasmid pPB1 from Plectonema boryanum. These observations suggest that such small cryptic plasmids as pPF1 and pPB1, both from Gram-negative and filamentous cyanobacteria, replicate by a rolling circle mechanism in their living cells.
