Ashikaga T, Wang Z, Yamamoto M, Yamasaki M, Magae J, Nagai K
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Biosci Biotechnol Biochem. 1994 May;58(5):839-42. doi: 10.1271/bbb.58.839.
Mouse macrophage (Mphi) hybridoma clones were generated by somatic cell hybridization of myeloma X63 cells (H-2d) with C57BL/6 (H-2b) peritoneal exudate cells elicited with a streptococcal preparation, OK432, or thioglycollate medium. Although they hardly adhered to plastic dishes and could not be morphologically distinguished from parental X63 tumor cells, the clones retained Mphi characteristics. These included phagocytosis and production of lysozyme and nonspecific esterase, suggesting that they were hybridomas derived from Mphi. Some of them expressed various levels of Ia antigen and Fc receptor. Because they induced proliferation of T cells from Balb/c mice but not those from C57BL/6 mice, the Ia antigen of Mphi hybridoma was assumed to be derived from peritoneal Mphi. The level of proliferation induction was correlated to the level of Ia antigen expression. Several clones produced a factor that cytostatically inhibited growth of murine mammary carcinoma and was serologically identified with arginine deiminase.
通过骨髓瘤X63细胞（H-2d）与经链球菌制剂OK432或巯基乙酸盐培养基诱导的C57BL/6（H-2b）腹膜渗出细胞进行体细胞杂交，产生了小鼠巨噬细胞（Mphi）杂交瘤克隆。尽管它们几乎不粘附于塑料培养皿，并且在形态上无法与亲代X63肿瘤细胞区分开来，但这些克隆保留了Mphi的特性。这些特性包括吞噬作用、溶菌酶和非特异性酯酶的产生，这表明它们是源自Mphi的杂交瘤。其中一些表达了不同水平的Ia抗原和Fc受体。由于它们能诱导Balb/c小鼠的T细胞增殖，但不能诱导C57BL/6小鼠的T细胞增殖，因此推测Mphi杂交瘤的Ia抗原源自腹膜Mphi。增殖诱导水平与Ia抗原表达水平相关。几个克隆产生了一种因子，该因子能抑制小鼠乳腺癌的生长，并且通过血清学鉴定为精氨酸脱亚氨酶。
