耐碱芽孢杆菌属YA-14果胶酸裂解酶基因的核苷酸序列
Nucleotide sequence of the pectate lyase gene from alkali-tolerant Bacillus sp. YA-14.
作者信息
Kim J M, Park H K, Yum D Y, Hahm B K, Bai D H, Yu J H
机构信息
Department of Food and Biotechnology, Yonsei University, Seoul, Korea.
出版信息
Biosci Biotechnol Biochem. 1994 May;58(5):947-9. doi: 10.1271/bbb.58.947.
The nucleotide sequence of the pectate lyase gene (pe lK) from alkali-tolerant Bacillus sp. was identified and analyzed. A 1,260-base pair open reading frame for the pe lK gene was observed and encoded for a protein of 420 amino acids. The signal peptide was composed of 21 amino acid residues. In the deduced primary structure of this enzyme, the three conserved regions of several pectate lyases were found and showed high homologies.
对耐碱芽孢杆菌属的果胶酸裂解酶基因（pelK）的核苷酸序列进行了鉴定和分析。观察到pelK基因有一个1260个碱基对的开放阅读框，编码一个由420个氨基酸组成的蛋白质。信号肽由21个氨基酸残基组成。在该酶推导的一级结构中，发现了几种果胶酸裂解酶的三个保守区域，且具有高度同源性。
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