水稻细胞悬浮培养物中编码I类几丁质酶的cDNA克隆的分离与鉴定

Isolation and characterization of cDNA clones encoding class I chitinase in suspension cultures of rice cell.

作者信息

Kim Y K, Baek J M, Park H Y, Choi Y D, Kim S I

机构信息

Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.

出版信息

Biosci Biotechnol Biochem. 1994 Jun;58(6):1164-6. doi: 10.1271/bbb.58.1164.

DOI:10.1271/bbb.58.1164

PMID:7765038

Abstract

We have cloned and determined the nucleotide sequences of full-length cDNA clones for chitinase, CH6 and CH16, whose sizes were about 1.1 kb with signal peptides, from rice cell suspension cultures. Northern blot analysis with CH16 as a probe showed that the chitinase transcript reached maximum at 8 h after elicitor-treatment and their sizes were about 1.1 kb, demonstrating that these clones were induced by elicitor.

摘要

我们从水稻细胞悬浮培养物中克隆并测定了几丁质酶CH6和CH16的全长cDNA克隆的核苷酸序列，其大小约为1.1 kb并带有信号肽。以CH16为探针进行的Northern印迹分析表明，几丁质酶转录本在诱导物处理后8小时达到最大值，其大小约为1.1 kb，这表明这些克隆是由诱导物诱导产生的。