Yu M, de Swaan Arons J, Smit J A
Faculty of Chemical Technology and Material Science, Delft University of Technology, The Netherlands.
J Chem Technol Biotechnol. 1994 Aug;60(4):413-8. doi: 10.1002/jctb.280600412.
A simple scaling analysis of protein solubility in semi-diluted polymer aqueous solutions was derived based on the recent theoretical development of Abbott et al. on the interactions between protein spheres and entangled polymer coils and the experimental observations of Atha and Ingham on the polymer-induced protein precipitation. Both the hard-sphere nature of the protein and the flexibility of the polymer coils have been taken into account in the model. The experimental data can be fitted by using this model with physically reasonable values of the exponent. This approach may be useful in estimating protein solubilities in water in the presence of polymer from limited and usually costly experimental data.
基于阿博特等人最近关于蛋白质球与缠结聚合物线圈之间相互作用的理论发展以及阿萨和英厄姆关于聚合物诱导蛋白质沉淀的实验观察结果，得出了蛋白质在半稀释聚合物水溶液中溶解度的简单标度分析。该模型考虑了蛋白质的硬球性质和聚合物线圈的柔韧性。使用该模型并结合具有物理合理指数值的数据，可以拟合实验数据。这种方法可能有助于从有限且通常成本高昂的实验数据中估算在聚合物存在下蛋白质在水中的溶解度。
