Waterhouse R N, Glover L A
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
Lett Appl Microbiol. 1994 Aug;19(2):88-91. doi: 10.1111/j.1472-765x.1994.tb00912.x.
Bioluminescence from developing L-form colonies of the plant pathogen, Pseudomonas syringae pv. phaseolicola, was monitored using the enhanced light-detecting capabilities of a charge-coupled device. During L-form induction, the bacteria entered a prolonged period during which the level of light output and hence metabolic activity, was very low. A relatively small number of highly bioluminescent L-form colonies were then observed to develop against a background of non-bioluminescent bacteria. When these colonies were sub-cultured and examined microscopically, typical L-form morphology was observed and continued high bioluminescence was detectable from derived colonies.
利用电荷耦合器件增强的光检测能力，对植物病原菌丁香假单胞菌菜豆致病变种发育中的L型菌落的生物发光进行了监测。在L型诱导过程中，细菌进入了一个延长的时期，在此期间光输出水平以及代谢活性都非常低。然后观察到在非生物发光细菌的背景下出现了相对少量的高生物发光L型菌落。当这些菌落进行传代培养并在显微镜下检查时，观察到典型的L型形态，并且从衍生菌落中可检测到持续的高生物发光。
