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Restriction analysis of an amplified polygalacturonase gene fragment differentiates strains of the phytopathogenic bacterium Pseudomonas solanacearum.

作者信息

Gillings M, Fahy P, Davies C

机构信息

Biological and Chemical Research Institute, Rydalmere, NSW, Australia.

出版信息

Lett Appl Microbiol. 1993 Jul;17(1):44-8. doi: 10.1111/j.1472-765x.1993.tb01432.x.

DOI:10.1111/j.1472-765x.1993.tb01432.x
PMID:7765308
Abstract

Amplification of a polygalacturonase gene fragment using the polymerase chain reaction (PCR) formed a rapid, sensitive and portable method for detecting and differentiating strains of Pseudomonas solanacearum, a taxonomically complex bacterial species. Primers 5'CAG CAG AAC CCG CGC CTG ATC CAG 3' and 5'ATC GGA CTT GAT GCG CAG GCC GTT 3' were used to amplify a 504 base pair polygalacturonase gene fragment from 57 Ps. solanacearum isolates. Digestion of these products with Hae III defined groups which reflected the known genetic divisions within the species.

摘要

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