Uesaka T, Yano K, Yamasaki M, Ando M
Laboratory of Physiology, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
Zoolog Sci. 1994 Jun;11(3):491-4.
Isolating a new somatostatin-like peptide from eel gut, the active residues to potentiate somatostatin action in the eel intestine were determined. In the newly-isolated peptide, the 5th or 6th Gln of the eel somatostatin (eSS-25II) was replaced with Glu. Although this peptide also enhanced the short-circuit current (Isc) across the intestine of the seawater eel, higher concentrations (approximately 30 fold) were required to obtain the same effects as eSS-25II. Since the Isc is due to active Cl- transport, this indicates that a structure formed by Gln-Gln residues at position 5 and 6 potentiates the somatostatin action to enhance ion and water transport across the intestine of the eel.
从鳗鱼肠道中分离出一种新的类生长抑素肽后，确定了在鳗鱼肠道中增强生长抑素作用的活性残基。在新分离的肽中，鳗鱼生长抑素（eSS - 25II）的第5或第6位谷氨酰胺被谷氨酸取代。尽管这种肽也增强了海水鳗鱼肠道的短路电流（Isc），但需要更高的浓度（约30倍）才能获得与eSS - 25II相同的效果。由于Isc是由活跃的氯离子转运引起的，这表明在第5和第6位由谷氨酰胺 - 谷氨酰胺残基形成的结构增强了生长抑素促进鳗鱼肠道离子和水运输的作用。
